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Otolaryngology, ENT & Sinus News Provided by the California Sinus CentersTime-Resolved Fluorescence Spectroscopy as a Diagnostic Technique of Oral Carcinoma: Validation in the Hamster Buccal Pouch Model [Original Article]February 1, 2010, 12:00 pm by Archives of Otolaryngology Head & Neck SurgeryObjective To investigate the benefit of using time-resolved, laser-induced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity. Design The carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) was applied to 1 cheek pouch of 19 hamsters. The contralateral pouch and the cheek pouches of 3 hamsters without DMBA exposure served as controls. Setting University of California, Davis. Participants Twenty-two golden/Syrian hamsters. Intervention A nitrogen pulse laser was used to induce tissue autofluorescence between the wavelengths of 360 and 650 nm. Main Outcome Measures Spectral intensities and time-domain measurements were obtained and compared with the histopathologic findings at each corresponding site. Results Spectral intensities and lifetime values at 3 spectral bands (SBs; SB1 = 380 ± 10 nm; SB2 = 460 ± 10 nm, and SB3 = 635 ± 10 nm) allowed for discrimination among healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma. The lifetime values at SB2 were the most important when distinguishing the lesions using only time-resolved parameters. An algorithm combining spectral fluorescence parameters derived from both spectral and time-domain parameters (peak intensities, average fluorescence lifetimes, and the Laguerre coefficient [zero-order]) for healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma provided the best diagnostic discrimination, with 100%, 100%, 69.2%, and 76.5% sensitivity and 100%, 92.2%, 97.1%, and 96.2% specificity, respectively. Conclusions The addition of time-resolved fluorescence-derived parameters significantly improves the capability of fluorescence spectroscopy–based diagnostics in the hamster buccal pouch. This technique provides a potential noninvasive diagnostic instrument for head and neck cancer.
More from Archives of Otolaryngology Head & Neck Surgery Long-term Health-Related Quality of Life in Survivors of Head and Neck Cancer [Original Article] Archives of Otolaryngology Head & Neck Surgery: January 16, 2012, 12:00 pm Objective To examine health-related quality of life (HRQOL) reported by 5-year head and neck cancer survivors and factors that predicted these long-term scores. Design Prospective, observational outcomes study. Setting Tertiary care institution. Patients A total Sequential Bilateral Cochlear Implantation in Children: Quality of Life [Original Article] Archives of Otolaryngology Head & Neck Surgery: January 16, 2012, 12:00 pm Objective To assess the effect of sequential bilateral cochlear implantation in children on their quality of life (QoL). Design Prospective cohort-control study. Setting Tertiary academic referral center. Patients Thirty children with prelingual deafness underwent sequential Disinfection of Flexible Fiberoptic Laryngoscopes After In Vitro Contamination With Staphylococcus aureus and Candida albicans [Original Article] Archives of Otolaryngology Head & Neck Surgery: January 16, 2012, 12:00 pm Objective To determine the efficacy of various cleaning and disinfective methods in reducing bacterial and fungal load on flexible fiberoptic laryngoscopes (FFLs). Design In vitro model. Subjects Flexible fiberoptic laryngoscopes contaminated with Staphylococcus aureus Winter scene, Pittsburgh, Pennsylvania [About the Cover] Archives of Otolaryngology Head & Neck Surgery: January 1, 2012, 12:00 pm About This Journal [About This Journal] Archives of Otolaryngology Head & Neck Surgery: January 1, 2012, 12:00 pm
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